Direct RT-qPCR andiany
Direct PCR
Inona no atao hoe Direct PCR
Direct PCR dia fomba fampitomboana ny ADN mivantana avy amin'ny ra, tavy biby, rambony voalavo, sela, sofina voalavo, trondro zebra, atodin'ny trondro, ravin-javamaniry, voa na santionany biolojika hafa tany am-boalohany nefa tsy manao ny fitokana-monina sy ny fanadiovana ny ADN.Ny teknika PCR mivantana dia afaka mampihena be ny fotoana andrana, sy ny fandaniana amin'ny tetikasa genotyping sy be dia be.Izy io koa dia manome safidy tsara kokoa rehefa miatrika fanamby amin'ny fampitomboana santionany faran'izay kely izay mety hitarika amin'ny fahaverezan'ny santionany ny dingana fanadiovana.
Ny PCR Mix FOREGENE dia manana fanoherana mafy amin'ny adin-tsaina, noho ny tanjaky ny FOREGENE Taq enzyme (patente nahazo alalana: ZL20161034512.0 (Shina), EP3450560 (Eoropeana), PCT/CN2017/082154 (Etazonia), patanty 6894926 (Japon)).Ny enzyme Taq-ny dia mampiasa ny teknolojia fampifandraisana ADN mba hanambatra indray ny faritra firafitry ny ADN amin'ny proteinina mifamatotra amin'ny asidra nukleika archaeal miaraka amin'ny polymerase ADN Taq mahatohitra hafanana mba hananganana ADN môdely miaraka amin'ny fifandraisana mihamitombo.Taq fanombohana mafana nohatsaraina izay mitazona ny fiasa isan-karazany amin'ny polymerase ADN tany am-boalohany. Ity enzyme Taq ity dia afaka mamatotra amin'ny ADN môdely ary manomboka ny synthesis ADN amin'ny môdely misy fifantohana ambany sy tontolo sarotra.
Ohatra, mora toy ny manampy ny sela, zavamaniry (ravina tanora, faka na voa) na santionan'ny biby amin'ny buffer, ary manampy ny master tsy manam-paharoa miaraka amin'ny primer manokana ao amin'ny fantsona PCR ary mandeha amin'ny alàlan'ny cycler mafana.Ny fahombiazan'ny kitapo dia mety amin'ny famakafakana santionany midadasika izay tena zava-dehibe ny fitsitsiana fotoana.
Ny rafitra fanehoan-kevitra PCR misy anzima UNG sy dUTP dia nofantenana araka ny fepetra andrana, izay afaka misoroka tsara ny loto ateraky ny vokatra fanamafisam-peo PCR.
Tombontsoa nyDirect PCR
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| Direct PCR | Fomba nentim-paharazana (fanadiovana RNA + PCR mahazatra) |
Fanjifana fitaovana | Fanjifana tavy | Kely kokoa | be |
Fanomanana modely | Karazana fitaovana | Tsy mila fitotoana | Mitoto santionany |
hetsika | Karazana fantsona tokana,10min | fandidiana sarotra, 1h | |
marary mitsika | 1-96 | 1-24 | |
PCR fanehoan-kevitra | rafitra fanehoan-kevitra | Optimized 2 × PCR mifangaro | Dntp, MgCl2,10 × PCR Buffer, Taq enzyme, |
Application
01 Famantarana ny microorganisms pathogenic
02 Famantarana novaina génétique, genotyping
03 Multiplex PCR / SNP detection / PCR-RFLP
04Sequencing/cloning
Product Series
ANDIAN-DAHATSORATRA | Anaran'ny vokatra | fepetra arahana | Catalog Number | Fepetra fitahirizana |
Direct RT-qPCR Series | 200T | DRT-01011 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
1000T | DRT-01012 |
ANDIAN-DAHATSORATRA | Anaran'ny vokatra | fepetra arahana | Catalog Number | Fepetra fitahirizana |
Blood Direct PCR Series | 200 × 20μl rxns | TP-04111 | -20 ℃ | |
2000 × 20μl rxns | TP-04113 | |||
200 × 20μl rxns | TP-04121 | -20 ℃ | ||
2000 × 20μl rxns | TP-04123 | |||
200 × 20μl rxns | TP-04211 | -20 ℃ | ||
2000 × 20μl rxns | TP-04213 | |||
200 × 20μl rxns | TP-04221 | -20 ℃ | ||
2000 × 20μl rxns | TP-04223 |
Series | Anaran'ny vokatra | fepetra arahana | Catalog Number | Fepetra fitahirizana |
Animal Direct PCR Series | Kit PCR mivantana tavy biby (65 ℃ lysis) | 200 × 20μl rxns | TP-01111 | Fizarana I 4 ℃, Fizarana II -20 ℃ |
2000 × 20μl rxns | TP-01113 | |||
Kit PCR mivantana tavy biby (65 ℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01123 | |||
Mouse Tail SuperDirectTMPCR Kit (lyse maripana efitra) | 200 × 20μl rxns | TP-01311 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01313 | |||
Kit PCR mivantana rambo totozy (65 ℃ lysis) | 200 × 20μl rxns | TP-01331 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01333 | |||
Kit PCR mivantana Rambo totozy (65 ℃ lysis) - UNG | 200 × 20μl rxns | TP-01341 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01343 | |||
Zebra Fish Direct PCR Kit (65 ℃ lysis) | 200 × 20μl rxns | TP-01411 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01413 | |||
Zebra Fish Direct PCR Kit(65 ℃ lysis) - UNG | 200 × 20μl rxns | TP-01421 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-01423 |
ANDIAN-DAHATSORATRA | Anaran'ny vokatra | fepetra arahana | Catalog Number | Fepetra fitahirizana |
Plant Direct PCR Series | 200 × 20μl rxns | TP-02111 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-02113 | |||
200 × 20μl rxns | TP-02121 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-02123 | |||
Fitaovana PCR Plus Direct Leaf Plant | 200 × 20μl rxns | TP-02131 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-02133 | |||
Plant Leaf Direct PCR Plus Kit -UNG | 200 × 20μl rxns | TP-02141 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-02143 | |||
200 × 20μl rxns | TP-03111 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-03113 | |||
200 × 20μl rxns | TP-03121 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-03123 | |||
200 × 20μl rxns | TP-03131 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-03133 | |||
200 × 20μl rxns | TP-03141 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-03143 | |||
Mamboly voa Direct PCR Plus Kit I (Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03151 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-03153 | |||
Voankazo Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03161 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-03163 | |||
200 × 20μl rxns | TP-03171 | Fizarana I 4 ℃, Fizarana II -20 ℃ | ||
2000 × 20μl rxns | TP-03173 | |||
Voan'ny zavamaniry Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03181 | Fizarana I 4 ℃, Fizarana II -20 ℃ | |
2000 × 20μl rxns | TP-03183 |